Journal: Frontiers in Immunology

Article Title: A Human Lin − CD123 + CD127 low Population Endowed with ILC Features and Migratory Capabilities Contributes to Immunopathological Hallmarks of Psoriasis

doi: 10.3389/fimmu.2017.00176

Figure Lengend Snippet: A human Lin − CD123 + CD127 low CLA + population with innate lymphoid cells (ILC) features in peripheral blood . (A) Compared expression of multiple ILC markers in the Lin − CD123 + CD127 low and Lin − CD127 + populations: black: Lin − CD123 + , gray: Lin − CD127 + , and white: isotype. Median fluorescence intensity values representative of three independent experiments are shown. (B) Inhibitor of DNA binding 2 (Id2) expression in sorted Lin − CD123 + from the lymphoid region (gated as shown in Figure S1A in Supplementary Material) (C) isotype and (D) Lin − CD127 + cells, (E) isotype, immunofluorescence staining for Id2 (green) and DAPI (nucleus staining), n = 10. (F) Nuclear median fluorescence intensity in Lin − CD123 + and Lin − CD127 + compared with the isotype control (*** p < 0.0001).

Article Snippet: The morphology of total pre-enriched Lin − CD123 + cells was evaluated in PBMCs previous to depletion of Lineage + cells (as described above) and stained with anti-CD123 and the nuclear dye DAPI (Thermo Fisher Scientific, MA, USA).

Techniques: Expressing, Fluorescence, Binding Assay, Immunofluorescence, Staining, Control

Journal: Mrs Bulletin

Article Title: Functional imaging of brain organoids using high-density microelectrode arrays

doi: 10.1557/s43577-022-00282-w

Figure Lengend Snippet: Human cerebral organoids comprise diverse cell types relevant to the study of neuronal circuit development and physiology. (a) Immunohistochemistry (IHC) revealed the presence of mature neurons (Tau/NeuN), astrocytes (GFAP), and oligodendrocytes (OLIG2) after 100 days of differentiation; nuclei were stained with DAPI (in blue). Scale bars are 50 μm. (b) Quantification of NeuN-positive cells. The ratio of the number of NeuN-positive cells to the total number of cells was quantified for both hCO lines (3 hCOs per line, for each hCO > 5000 cells). The ratio of NeuN-positive cells to total cells did not differ significantly between the two lines. (c) Single-cell RNA sequencing (scRNA-seq) was performed to quantify the cellular composition of hCOs and their comparability. The results are summarized in two pie charts with the identified cell type fractions displayed for each line. For both lines the results confirmed the presence of dopaminergic, GABAergic, glutamatergic and cholinergic neurons, neural crest cells (NCCs), radial glia (RG), astrocytes and oligodendrocytes.

Article Snippet: After washing three times with PBS, sections were incubated with PureBlu DAPI (Bio-Rad, Hercules, CA, USA, #1351303) for 3 min and mounted with ProLong Gold antifade mounting medium (Thermo Scientific, #P36930).

Techniques: Immunohistochemistry, Staining, RNA Sequencing